human rtk phosphorylation antibody array 1  (RayBiotech inc)

Journal: International Journal of Molecular Sciences

Article Title: ANGPTL4 Induces TMZ Resistance of Glioblastoma by Promoting Cancer Stemness Enrichment via the EGFR/AKT/4E-BP1 Cascade

doi: 10.3390/ijms20225625

Figure Lengend Snippet: ANGPTL4-regulated phosphorylation cascade for GSC enrichment. ( A ) The putative target receptor of ANGPTL4 was detected by Human receptor tyrosine kinase (RTK) Phosphorylation Antibody Array C1. ( B ) The putative phosphorylation signaling pathway of ANGPTL4 was detected by Human and Mouse AKT Pathway Phosphorylation Array C1. ( C ) Epidermal growth factor receptor (EGFR) phosphorylation (Y1068) and Eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1; Thr37/46) phosphorylation in A172 and U87MG were validated by Western blotting. ( D ) After ANGPTL4 overexpression for 24 h, cells were treated with gefitinib for an additional 24 h. The protein expression was validated by Western blotting. The experiment was performed independently 3 times, and quantitative results were expressed as the mean ± SEM (* p < 0.05). ( E ) After ANGPTL4 overexpression for 24 h, cells were treated with the AKT or PI3K inhibitor for another 24 h. The protein expressions were validated by Western blotting. The experiment was performed independently three times, and quantitative results were expressed as the mean ± SEM (* p < 0.05).

Article Snippet: Human RTK Phosphorylation Antibody Array C1 (AAH-PRTK-1-4) and Human AKT Pathway Phosphorylation Array C1(AAH-AKT-1-4) (RayBiotech, Inc., Norcross, GA, USA) were used to detect the signaling cascade activated by ANGPTL4.

Techniques: Ab Array, Binding Assay, Western Blot, Over Expression, Expressing